The COBAS® AmpliPrep Total Nucleic Acid Isolation Kit (TNAI) is designed for the preparation of highly purified total nucleic acid from serum or plasma (collected in the anticoagulants EDTA or ACD only) on the COBAS® AmpliPrep Instrument.
The purified nucleic acid can be used in any nucleic acid amplification technology (e.g. in combination with COBAS® TaqMan® Analysers or the LightCycler® instruments). An Internal Control or Quantitation Standard (IC/QS) can be incorporated into each individual sample and is carried through the sample preparation. It compensates for loss of target and traces of potential inhibitors in the consecutive amplification step. It is the user’s responsibility to validate the performance of TNAI including the use of an IC/QS for any particular use.